vestarВестник аритмологииJournal of Arrhythmology1561-86412658-7327НАО «Инкарт»10.35336/VA-1167vestar-1274Research ArticleЭКСПЕРИМЕНТАЛЬНЫЕ ИССЛЕДОВАНИЯEXPERIMENYAL ARTICLESМетодика лапароскопической ренальной денервации: пилотное исследование на животныхLaparoscopic approach for renal denervation: an experimental study on animalshttps://orcid.org/0000-0002-0455-2422БаштаД. И.BashtaD. I.

Башта Денис Игоревич.

Пятигорск, просп. Калинина, д. 33

Denis I. Bashta.

Pyatigorsk, 33 Kalinin str.

surgeondenis@mail.ruhttps://orcid.org/0000-0002-8443-015XВиленскийЛ. И.VilenskiyL. I.

Ставрополь, ул. Пригородная, д. 224-а

Stavropol, 224-a Prigorodnaya str.

https://orcid.org/0000-0001-7869-6958КривошеевЮ. С.KrivosheevYu. S.

Ставрополь, ул. Пригородная, д. 224-а

Stavropol, 224-a Prigorodnaya str.

https://orcid.org/0000-0002-6498-7399МодниковК. В.ModnikovK. V.

Ставрополь, ул. Пригородная, д. 224-а

Stavropol, 224-a Prigorodnaya str.

https://orcid.org/0000-0003-1439-8585МодниковаМ. А.ModnikovaM. A.

Ставрополь, ул. Пригородная, д. 224-а

Stavropol, 224-a Prigorodnaya str.

https://orcid.org/0000-0002-6958-6690РомановА. Б.RomanovA. B.

Новосибирск, ул. Речкуновская, д. 15

Novosibirsk, 15 Rechkunovskaya str.

ГБУ «Городская клиническая больница»РоссияSBIH «City Сlinical Hospital»Russian FederationГБУЗ «Краевой клинический кардиологический диспансер» Ставропольского краяРоссияSBIH «Regional clinical cardiologic hospital» of Stavropol regionRussian FederationФГБУ «НМИЦ имени академика Е.Н. Мешалкина» МЗ РФРоссияFSBI «Meshalkin National Medical Research Centre» of the Ministry of Health of RussiaRussian Federation2023191020233046168Copyright © Башта Д.И., Виленский Л.И., Кривошеев Ю.С., Модников К.В., Модникова М.А., Романов А.Б., 20232023Башта Д.И., Виленский Л.И., Кривошеев Ю.С., Модников К.В., Модникова М.А., Романов А.Б.Bashta D.I., Vilenskiy L.I., Krivosheev Y.S., Modnikov K.V., Modnikova M.A., Romanov A.B.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestar.elpub.ru/jour/article/view/1274Цель

Цель. Оценить эффективность и безопасность лапароскопической механической ренальной денервации.

Материал и методы исследования. Тридцать овец весом 92,3±2,1 кг разделены на 2 группы: контрольную (группа I, n=15) и механической ренальной денервации (группа II, n=15). Период послеоперационного наблюдения составлял 6 месяцев. В ходе исследования оценивались следующие показатели: артериальное давление (АД) в ответ на высокочастотную электрическую стимуляцию (ВЭС) из просвета сосуда, креатинин и мочевина, ятрогенные повреждения почечных артерий по результату ангиографии, степень разрушения нервных волокон по данным гистологического исследования.

Результаты

Результаты. За весь период наблюдения - все животные живы. Значения мочевины и креатинина в течение исследования не выходили за границы нормы. Стенотических ятрогенных повреждений ангиография почечных артерий не выявила. Через 6 месяцев во 2-й группе отмечается гипертензивный ответ АД на ВЭС, так же, как и в контрольной (повышение систолического АД ∆70,27±5,31 мм рт. ст. (p>0,005), диастолического АД ∆49,87±6,04 мм рт. ст. (p>0,005). Через 6 месяцев животные выводились из исследования для гистологического анализа. Гистологическое исследование показало: во второй группе имелись отечность и вакуолизация нервного волокна без признаков демиелинизации, полного разрушения нервного волокна не наблюдалось.

Выводы

Выводы. Лапароскопическая механическая ренальная денервация является безопасной методикой. Гипотензивный результат лапароскопической механической ренальной денервации был краткосрочным.

Aim

Aim. To study the efficacy and safety of laparoscopic mechanical renal denervation.

Methods

Methods. Thirty sheep with mean weight 92.3±2.1 kg were divided in 2 groups: control group (group I, n=15) and mechanical laparoscopic renal denervation group (group II, n=15). The follow-up period was 6 months. The following parameters were assessed: blood pressure in response to high-frequency electrical intraluminal stimulation, creatinine, blood urea nitrogen test (BUN), damage to the renal arteries according to the result of angiography, the degree of destruction of nerve fibers according to histological examination.

Results

Results. All animals were alive during the entire observation period. The creatinine and BUN were within the normal ranges during the observation period. Angiography of the renal arteries did not reveal iatrogenic stenotic lesions. After 6 months in group 2 there was hypertensive response of blood pressure to high-frequency electrical stimulation (in-creased systolic blood pressure ∆70.27±5.31 (p>0.005), diastolic blood pressure ∆49.87±6.05 (p>0.005)). After 6 months, the animals were euthonised, further histological examination was completed. Histological examination: in the group II, there was swelling and vacuolization of the nerve fiber without signs of demyelination.

Conclusions

Conclusions. Laparoscopic mechanical renal denervation is a safe technique. However, the hypotensive effect is short-term.

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The authors declare that there are no conflicts of interest present.